DNA and Forensics: Look How Far We’ve Come

July 29, 2010

This week is the anniversary of the first fingerprint being taken and we’re reminded how far forensic science has evolved. Forensics, the study of evidence that identifies a subject, usually refers to identifying a victim or subject of interest at a crime scene.

Forensic scientists use a variety of techniques for identification, however one that is particularly helpful is DNA profiling. This involves utilizing bodily fluids, hair, or skin particles to identify an individual. In addition to DNA profiling, forensics looks at the other evidence to make certain that the identity of a subject is accurate.

Electrophoresis

Gel XL Ultra V-2 Electrophoresis System

Electrophoresis techniques allow the identification of DNA sequences. Cole-Parmer offers a complete line of products for electrophoresis including horizontal and vertical gel electrophoresis systems, reagents, imaging systems, and power supplies.

Another forensic method is polymerase chain reaction (or PCR). In PCR, a strand of DNA is amplified and replicated through a series of temperature cycles. PCR requires the use of a temperature stable DNA polymerase that replicates the DNA through an enzymatic process that also requires DNA primers, deoxynucleoside triphosphates, and a suitable buffer solution. Cole-Parmer also offers products for polymerase chain reaction (PCR). Products include thermal cyclers; chilling, heating and mixing blocks; PCR tubes and strips; PCR plates; hybridization incubators; and sample counters.

Pict: Gel XL Ultra V-2 Electrophoresis System


Summer Blockbuster from Masterflex® (or Viral Video Hit)?

July 22, 2010

Now playing at Coleparmer.com or a Youtube channel near you:  How to load Masterflex Tubing Pump Heads.   

Ok, our latest videos may not be this summer’s blockbuster movie or the latest viral video on Youtube, but they are informative.     


  

Our popular line of Masterflex peristaltic pumps offer tremendous advantages in applications requiring liquid handling or pumping precise volumes of fluids at consistent flow rates.  Now, our latest videos illustrate how to quickly and easily load tubing into our Masterflex L/S® Standard Pump Heads and Masterflex I/P® High-Performance Tubing pump heads.  These videos offer time-saving tips and address questions we commonly receive from customers.      

Masterflex L/S Tubing Pumps

Masterflex L/S Tubing Pumps

Masterflex L/S pumps include fixed- and variable-speed pumps. Masterflex L/S tubing pumps feature high-accuracy drives for precise flow control and are ideal for laboratory, field, or process applications.      

Masterflex I/P Performance Tubing Pumps

Masterflex I/P Tubing Pumps

Masterflex I/P peristaltic tubing pumps offer flow ranges from 0.012 to 17 liters per minute. Masterflex I/P pumps are characterized by heavier motors and better protection from the hazards of industry such as dust and water. Use these pumps in your manufacturing process or for quick fluid transfer in your lab.      


For Ice Cream and Other Treats, Choose the Right Mixer

July 15, 2010
Cole-Parmer® Stir-Pak® General Purpose Laboratory Mixers

Cole-Parmer® Stir-Pak® General Purpose Laboratory Mixers

  

With July as National Ice Cream Month, we turn our attention to some savvy equipment that can help prepare delicious treats. Specifically, today’s focus is mixers. Cole-Parmer offers a broad selection of mixers and mixer products for various applications. Selecting an appropriate mixer is easy once you consider the variables:         

  • Container capacity: tank diameter and batch height
  • Liquid viscosity: different types of liquids display different characteristics when force is applied. Four most common types of liquid behavior are listed below—all of our mixers are to be used with fluids exhibiting Newtonian, pseudoplastic, or thixotropic behaviors.
  • –Dilatant Liquids—viscosity increases as shear rate increases. Mixers can bog down and stall after initially mixing such liquids. Dilatant liquids include slurries, clay, and candy compounds. View Real-Torque Digital Brushless Mixers.
     –Newtonian Liquids—viscosity remains constant regardless of shear rate or agitation. As mixer speed increases, flow increases proportionately. Newtonian liquids include water, mineral oils, and hydrocarbons. See the Cole-Parmer® Stir-Pak® General Purpose Laboratory Mixers.
     –Pseudoplastic Liquids—viscosity decreases as shear rate increases, but initial viscosity may be sufficiently great to prevent mixing. Typical pseudoplastic liquids are gels, latex paints, and lotions.
     –Thixotropic Liquids—as with pseudoplastic liquids, viscosity decreases as shear rate or agitation increases. When agitation is stopped or reduced, hysteresis occurs and viscosity increases. Often the viscosity will not return to its initial value. Thixotropic liquids include soaps, tars, shortening, glue, inks, and peanut butter. 
  • Torque requirements: the rotational force required of the mixer motor—measured in in-oz or in-lb. Torque and speed are inversely related (as speed increases, torque decreases).
  • Horsepower (hp) requirements: the efficiency required of the mixer motor with regard to torque (in-oz) and to the rotation speed (in revolutions per minute—rpm) as defined by the following equation: hp = (in-oz x rpm)/1,008,400
  • Rotational speed (rpm) and diameter of mixing propeller: A small increase in the rpm or diameter greatly increases the power required for mixing. This relationship can be expressed as follows: Power α rpm3 x dia5
  • Duty cycle: the time interval devoted to starting, running, stopping, and idling when a device is used intermittently.
  • Power supply: electric (115 or 230 VAC) or air-drive mixers for areas where electricity is unsafe or impractical.

See Cole-Parmer at IFT, Chicago, July 18-20

July 8, 2010

Take a look at Cole-Parmer’s new and best-selling food process, quality, and safety products at the IFT Annual Meeting & Food Expo®, held at McCormick Place in Chicago.

APEX 100 Metal Detector

APEX 100 Metal Detector

Innovative new products on display include the APEX 100 Metal Detector from Thermo Scientific. Using only food-grade materials in its construction, the APEX 100 meets the hygienic standards required by the industry—including HACCP policy compliance. It features dual frequency and gain operation for maximum application flexibility and is fully tested to meet IP65/NEMA/ATEX requirements. Designed for reliability and ease of use, the detector can be matched exactly to a specific conveyor width and product height through numerous aperture size configurations available.

Instruments for food quality and safety testing including refractometers, pH meters, viscometers, thermometers, and more will also be displayed.

Stop by booth #6719 and register for a chance to win one of twenty Super-Sizzling Summer Cool Packs containing a 12-can MusiCooler, a Wireless Weather Station, a 28-oz water bottle, and a freezer pack. You can also request the 2010 Cole-Parmer® FoodTECH Source® catalog.

For more information, call 800-323-4340 or visit ColeParmer.com/7979.


Holiday BBQ? Make Sure Your Meat Temperature is Safe

July 1, 2010
 
NSF-Flat-Surface Pocket Thermometer

NSF-Flat-Surface Pocket Thermometer

As the Fourth of July holiday nears, many are planning picnics and barbeques as they await the evening fireworks. Grilled brats, hamburgers, hot dogs, steak, fish, and chicken are typical fare for a summer feast. Yet, if meat is undercooked, it can lead to foodborne illness with symptoms including upset stomachs, diarrhea, fever, vomiting, cramps, dehydration, and more.       

To avoid ending the party before the light show begins, check the internal temperature of meat prior to serving it. The U.S. Food and Drug Administration recommends a temperature of at least 71 °C (160 °F) for ground beef and at least 63 °C (145 °F) for steaks and fish. Poultry requires even higher temperatures 74°C (165 °F). Temperatures should be measured in the thickest part of the meat.      

NSF-Flat-Surface Pocket ThermometerCole-Parmer offers several NSF-Approved Digital Pocket Thermometers ideal for checking food temperatures. The economical Basic Thermometer has a temperature range of -40 to 300 °F and features a data hold button to freeze readings. The Cole-Parmer® Thermometer has °F/°C switchable ranges and displays updates every second. The Taylor® Flat-Edged Dial Safe T-Guard Thermometer includes a microbial sleeve with an additive that inhibits bacterial and fungal growth. The Flat-Surface Thermometer  is ideal for measuring temperatures on cooking grills, motors, or other flat surfaces. Many more options are also available online.    

Make it a safe holiday. As you grill out, keep the temperatures rising.


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