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February 16, 2012

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Extracting DNA Samples

Challenge: Larry from Labotech Logistics is extracting DNA samples for forensic research. Since DNA is pH sensitive, the researchers are using Tris buffers to stabilize the pH of the samples he prepares. Recently they had a problem with the effectiveness of their buffers and lost several DNA samples. They can’t afford for this to happen again, so Larry wants to check the pH quality of his samples throughout the process.

Larry dusted off an older pH meter, but it was missing an electrode. What type of pH electrode should Larry purchase when working this type of application?

  1. A low-cost single-junction electrode
  2. Any combination pH electrode with built-in ATC (Automatic Temperature Compensation).
  3. A calomel (mercury chloride) electrode
  4. A double-junction electrode

See the answers.

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